Journal: Nucleic Acids Research

Article Title: RecJ exonuclease: substrates, products and interaction with SSB

doi: 10.1093/nar/gkj503

Figure Lengend Snippet: RecJ recognizes phosphorylated and non-phosphorylated 5′ ends. The 3′ 32 P-labeled oligonucleotide A was either phosphorylated with T4 polynucleotide kinase on the 5′ end or left unphosphorylated. ( A ) In a 20 µl reaction, 0.1 pmol of each substrate was added to various amounts of purified RecJ or, after annealing with equimolar complementary oligonucleotide B, λ exonuclease. ( B ) Phosphorimager analysis of three independent nuclease assays with RecJ and 5′ phosphorylated substrate (circles) and 5′ OH substrate (triangles).

Article Snippet: Bio-Rad Quantity One phosphorimager software was utilized to quantify degradation activity.

Techniques: Labeling, Purification